Testosterone, Total

WestSouthwest
  • Test Code: LAB2111222
  • Interface Code: 11289
Department: Toxicology
Last Updated: 12/12/2025

Synonyms

Testosterone by LCMS | Testosterone Total | 533 | SHO11289 | 1230101204;1230102806

Specimen Collection

Quick Collect

+S/PO/R

Collection Instructions

Specimen Collection: Blood

Container(s): 

CHW: Red top (no additive, PREFERRED), Gold top (SST-Gel, acceptable)

CHS: Mint Green Top (Lithium Heparin Gel) or Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 3.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok? Yes

Microtainer acceptable?  Yes

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: 
    • CHW: Serum
    • CHS: Plasma or Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processing Instructions:
    • Gold/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
    • Green/Plasma: Centrifuge after collection.
  • Minimum volume: 1.0 mL 
  • Transport Temperature: Refrigerate

Specimen Stability and Storage

Ambient: 72 hours

Refrigerate: 7 days

Frozen: 14 days

Laboratory Retention: 7 days

Performed

Batched and run 2 times per week, TAT 1-3 days.

Reference Range

Testosterone LCMS
Male
0 Minutes – 6 Months: 75 – 400 ng/dL
6 Months – 10 Years: 0 – 20 ng/dL
10 Years – 12 Years: 0 – 130 ng/dL
12 Years – 14 Years: 0 – 800 ng/dL
14 Years – 15 Years: 0 – 1200 ng/dL
15 Years – 17 Years: 100 – 1200 ng/dL
17 Years – 19 Years: 300 – 1200 ng/dL
19 Years – 150 Years: 240 – 950 ng/dL

Female
0 Minutes – 6 Months: 20 – 80 ng/dL
6 Months – 10 Years: 0 – 20 ng/dL
10 Years – 12 Years: 0 – 44 ng/dL
12 Years – 17 Years: 0 – 75 ng/dL
17 Years – 19 Years: 20 – 75 ng/dL
19 Years – 150 Years: 8 – 60 ng/dL

Performing Laboratory

CHW: Corewell Health Reference Laboratory, Grand Rapids, MI
CHS: Corewell Health Lakeland St. Joseph Laboratory, St. Joseph, MI

Methodology

Liquid Chromatography-Mass Spectrometry.

This test was developed and its performance characteristics determined by Spectrum Health Toxicology Laboratory. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

CPT Code

84403

CDM Code

3018440302

LOINC

Testosterne LCMS: 2986-8

Clinical Reviewed Date

3/25/2025