Testosterone, Free (Dialysis) and Total, MS

Send Outs
  • Test Code: LAB1232314
  • Interface Code: 1232314
Department: Send Outs
Last Updated: 2/20/2026

Synonyms

QUEST: 36170 | total test, free test, free and total test, testosterone, mass spec

Ordering Instructions

Includes: Testosterone, Total, MS and Testosterone, Free (Dialysis)

This assay is intended for
  • Assessing testicular function in prepubescent hypogonadal males
  • Management of hirsutism and virilization in females
  • Please note: This test is not appropriate for general testosterone screening.

 

Specimen Collection

Collection Instructions

Specimen Collection: Blood

 

Container(s): Gold Top (Serum Separator-SST Gel) or Red Top (no gel)

Minimum Volume to Collect: 3.0 mL

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours -- See Processing Instructions.

Processing Instructions

Laboratory, Outpatient or Off-site collection

Processing Instructions:

  • Specify age and sex on test request form.
  • Red-top tube (no gel): Allow blood to clot at room temperature. Centrifuge to separate the serum from the cells and immediately pour serum into a plastic transport tube.
  • Serum separator tube: Allow blood to clot at room temperature, centrifuge, and remove serum from the gel immediately (not to exceed 48 hours) by pouring serum into a plastic transport tube.

Processed Specimen: 1.8 mL Serum (Minimum volume: 0.9 mL)

Centrifuge/Spin: Yes

Aliquot: Yes

 

Transport Instructions

Transport Container: Transport tube

Transport Temperature: Room temperature

 

Rejection Criteria

Gross hemolysis • Grossly lipemic

Specimen Stability and Storage

 

  • Room temperature: 7 days
    Refrigerated: 21 days
    Frozen: 2 years

Performed

Quest Diagnostics Laboratory

Reference Range

See Laboratory Report

Clinical Information

Testosterone, Free (Dialysis) and Total, MS - Helpful in assessing testicular function in prepubescent hypogonadal males and in managing hirsutism, virilization in females.

Performing Laboratory

Quest Diagnostics 

Methodology

Chromatography/Mass Spectrometry • Equilibrium Dialysis • Calculation (CALC)

CPT Code

84402, 84403

Clinical Reviewed Date

2/18/2026