Testosterone Free and Total

WestSouthwest
  • Test Code: LAB173
  • Interface Code: 10257
Department: Toxicology
Last Updated: 1/22/2026

Synonyms

1230101205 | 84403, 84402

Specimen Collection

Quick Collect

+S/PO/R

Collection Instructions

Specimen Collection: Blood

Container(s):

CHW: Red top (no additive, PREFERRED), Gold top (SST-Gel, acceptable)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 3.0 mL

Neonate Volume to Collect: 3.0 mL

Capillary collect ok? Yes

Microtainer acceptable? Yes

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

 

Processing Instructions

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processing Instructions:
  • Gold or Red Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
  • Minimum volume: 1.5 mL
  • Transport Temperature: Refrigerate

 

Specimen Stability and Storage

Ambient: 72 hours

Refrigerate: 7 days

Frozen: 14 days

Laboratory Retention: 7 days

 

 

Reference Range

Free Testosterone

Male

16 Years – 150 Years: 9 – 30 ng/dL

Female

16 Years – 150 Years: 0.3 – 1.9 ng/dL

 

Total Testosterone.

Male

0 Minutes – 6 Months: 75 – 400 ng/dL

6 Months – 10 Years: 0 – 20 ng/dL

10 Years – 12 Years: 0 – 130 ng/dL

12 Years – 14 Years: 0 – 800 ng/dL

14 Years – 15 Years: 0 – 1200 ng/dL

15 Years – 17 Years: 100 – 1200 ng/dL

17 Years – 19 Years: 300 – 1200 ng/dL

19 Years – 150 Years: 240 – 950 ng/dL

 

Female

0 Minutes – 6 Months: 20 – 80 ng/dL

6 Months – 10 Years: 0 – 20 ng/dL

10 Years – 12 Years: 0 – 44 ng/dL

12 Years – 17 Years: 0 – 75 ng/dL

17 Years – 19 Years: 20 – 75 ng/dL

19 Years – 150 Years: 8 – 60 ng/dL

Clinical Information

This test was developed and its performance characteristics determined by Spectrum Health Toxicology Laboratory. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Performing Laboratory

Corewell Health Reference Laboratory, Grand Rapids, MI

 

Methodology

Liquid Chromatography-Mass Spectrometry

CPT Code

84403

84402

CDM Code

3018440302, 3018440201

Clinical Reviewed Date

3/25/2025